kpcczКомплексные проблемы сердечно-сосудистых заболеванийComplex Issues of Cardiovascular Diseases2306-12782587-9537Federal State Budgetary Institution “Research Institute for Complex Issues of Cardiovascular Diseases”10.17802/2306-1278-2024-13-3-200-214kpccz-1186Research ArticleОНЛАЙН. АНАЛИТИЧЕСКИЙ ОБЗОР. Патологическая физиологияДИАГНОСТИЧЕСКАЯ ЗНАЧИМОСТЬ ОПРЕДЕЛЕНИЯ ОТДЕЛЬНЫХ СУБПОПУЛЯЦИЙ ВНЕКЛЕТОЧНЫХ ВЕЗИКУЛ В КЛИНИЧЕСКОЙ ПРАКТИКЕDIAGNOSTIC POTENTIAL OF DETERMINING INDIVIDUAL EXTRACELLULAR VESICLES SUBSETS IN CLINICAL PRACTICEhttps://orcid.org/0000-0001-7204-7850КудрявцевИгорь ВладимировичKudryavtsevIgor V.

кандидат биологических наук заведующий лабораторией аутоиммунных и аутовоспалительных заболеваний научного центра мирового уровня «Центр персонализированной медицины» федерального государственного бюджетного учреждения «Национальный медицинский исследовательский центр имени В. А. Алмазова» Министерства здравоохранения Российской Федерации, Санкт-Петербург, Российская Федерация; заведующий лабораторией клеточной иммунологии федерального государственного бюджетного научного учреждения «Институт Экспериментальной Медицины», Санкт-Петербург, Российская Федерация

PhD, Head of the Laboratory of Autoimmune and Autoinflammatory Diseases, World-Class Science Center “Center for Personalized Medicine”, Federal State Budgetary Institution “V. A. Almazov National Medical Research Center” of the Ministry of Health of the Russian Federation, St. Petersburg, Russian Federation; Head of the Laboratory of Cellular Immunology, Federal State Budgetary Institution “Institute of Experimental Medicine”, St. Petersburg, Russian Federation

igorek1981@yandex.ruhttps://orcid.org/0000-0002-7577-628XГоловкинАлексей СергеевичGolovkinAlexey S.

доктор медицинских наук руководитель группы генно-клеточной инженерии института молекулярной биологии и генетики федерального государственного бюджетного учреждения «Национальный медицинский исследовательский центр имени В. А. Алмазова» Министерства здравоохранения Российской Федерации, Санкт-Петербург, Российская Федерация

PhD, Head of the Group of Genetic and Cellular Engineering at the Institute of Molecular Biology and Genetics, Federal State Budgetary Institution “V. A. Almazov National Medical Research Center” of the Ministry of Health of the Russian Federation, St. Petersburg, Russian Federation

golovkin_a@mail.ruhttps://orcid.org/0000-0003-4571-8799ТотолянАрег АртемовичTotolyanAreg A.

академик РАН, доктор медицинских наук, профессор директор федерального бюджетного учреждения науки «Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им. Пастера» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека, Санкт-Петербург, Российская Федерация

Academician of the Russian Academy of Sciences, PhD, Professor, Director of the Federal Budgetary Institution “Saint-Petersburg Pasteur Institute” of the Federal Service for the Oversight of Consumer Protection and Welfare, St. Petersburg, Russian Federation

totolian@pasteurorg.ruФедеральное государственное бюджетное учреждение «Национальный медицинский исследовательский центр имени В. А. Алмазова» Министерства здравоохранения Российской Федерации; Федеральное государственное бюджетное научное учреждение «Институт Экспериментальной Медицины»РоссияFederal State Budgetary Institution “V. A. Almazov National Medical Research Center” of the Ministry of Health of the Russian Federation; Federal State Budgetary Institution “Institute of Experimental Medicine”Russian FederationФедеральное государственное бюджетное учреждение «Национальный медицинский исследовательский центр имени В. А. Алмазова» Министерства здравоохранения Российской ФедерацииРоссияFederal State Budgetary Institution “V. A. Almazov National Medical Research Center” of the Ministry of Health of the Russian FederationRussian FederationФедеральное бюджетное учреждение науки «Санкт-Петербургский научно-исследовательский институт эпидемиологии и микробиологии им. Пастера» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человекаРоссияSaint-Petersburg Pasteur InstituteRussian Federation202430092024133202216Copyright © Кудрявцев И.В., Головкин А.С., Тотолян А.А., 20242024Кудрявцев И.В., Головкин А.С., Тотолян А.А.Kudryavtsev I.V., Golovkin A.S., Totolyan A.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.nii-kpssz.com/jour/article/view/1186Основные положения

Основные положения

В обзоре рассмотрены вопросы, связанные с диагностической ценностью определения уровня внеклеточных везикул, а также переносимого ими груза нуклеиновых кислот и белков, у пациентов с различными заболеваниями и патологическими состояниями. Оценены представленные в литературе данные о диагностической значимости отдельных видов микроРНК при злокачественных новообразованиях.

 

 

Аннотация

Аннотация

Накопление большого массива информации о биологической природе внеклеточных везикул, а также их участии во многих биологических и патологических процессах в организме предопределяет необходимость трансляции полученных знаний в практическую медицину. Очевидно, что на данном этапе наиболее перспективной представляется разработка диагностических и прогностических алгоритмов, основанных на исследовании уровня целевых внеклеточных везикул у пациентов при различных патологических состояниях. В данном обзоре предпринята попытка систематизации имеющихся данных об опыте и/или перспективах использования отдельных классов внеклеточных везикул в диагностике ряда наиболее распространенных заболеваний и патологических состояний (ишемические и реперфузионные повреждения тканей и органов, инфекционные и неинфекционные воспалительные заболевания, злокачественные опухоли). Отдельно рассмотрены потенциально применимые с диагностической целью внеклеточные везикулы – их белковый состав, а также репертуар переносимых нуклеиновых кислот (в первую очередь микроРНК).

Highlights

Highlights

The review is dedicated to analysis of available data on diagnostic significance of extracellular vesicles and their nucleic acids and proteins cargo. The analysis is focused on clinical application of testing systems in various pathological conditions and illnesses. In particular, diagnostic value of miRNA levels in different types of cancer are also presented.

 

 

Abstract

Abstract

The increased number of performed studies investigating biological nature of extracellular vesicles, as well as their participation in many biological and pathological processes, determines the need to translate the acquired knowledge into practical medicine. The development of diagnostic and prognostic algorithms based on the study of the level of target extracellular vesicles in patients with various pathological conditions is the most promising way of translation of this knowledge. This review attempts to systematize the available data on the experience and/or prospects for the use of certain classes of extracellular vesicles in the diagnosis of the most common diseases and pathological conditions (ischemic and reperfusion injuries of tissues and organs, infectious and non-infectious inflammatory diseases, malignant tumors, etc.). Potentially applicable extracellular vesicles for diagnostic purposes are considered separately in terms of their protein composition, as well as in terms of the repertoire of transferred nucleic acids (primarily miRNAs).

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The authors declare that there are no conflicts of interest present.