kpcczКомплексные проблемы сердечно-сосудистых заболеванийComplex Issues of Cardiovascular Diseases2306-12782587-9537Federal State Budgetary Institution “Research Institute for Complex Issues of Cardiovascular Diseases”10.17802/2306-1278-2018-7-2-25-36kpccz-420Research ArticleПЕРЕДОВАЯ СТАТЬЯEDITORIALIN SITU VASCULAR TISSUE REMODELING USING BIODEGRADABLE TUBULAR SCAFFOLDS WITH INCORPORATED GROWTH FACTORS AND CHEMOATTRACTANT MOLECULESIN SITU VASCULAR TISSUE REMODELING USING BIODEGRADABLE TUBULAR SCAFFOLDS WITH INCORPORATED GROWTH FACTORS AND CHEMOATTRACTANT MOLECULESAntonovaL. V.AntonovaL. V.

PhD, the Head of the Laboratory of Cell Technologies

PhD, the Head of the Laboratory of Cell Technologies

antonova.la@mail.ruSevostyanovaV. V.SevostyanovaV. V.

PhD, researcher at the Laboratory of Cell Technologies

PhD, researcher at the Laboratory of Cell Technologies

MironovA. V.MironovA. V.

research assistant at the Laboratory of Cell Technologies

research assistant at the Laboratory of Cell Technologies

KrivkinaE. O.KrivkinaE. O.

research assistant at the Laboratory of Cell Technologies

research assistant at the Laboratory of Cell Technologies

VelikanovaE. A.VelikanovaE. A.

PhD, researcher at the Laboratory of Cell Technologies

PhD, researcher at the Laboratory of Cell Technologies

MatveevaV. G.MatveevaV. G.

PhD, senior researcher at the Laboratory of Cell Technologies

PhD, senior researcher at the Laboratory of Cell Technologies

GlushkovaT. V.GlushkovaT. V.

PhD, researcher at the Laboratory of Novel Biomaterials

PhD, researcher at the Laboratory of Novel Biomaterials

ElgudinYa. L.ElgudinYa. L.

MD, PhD, Assistant Professor, Surgery, Case Western Reserve University School of Medicine, Cleveland, Ohio, United States of America; Chief, Division of Cardiothoracic Surgery Louis Stokes Cleveland VA Medical Center Cleveland, Ohio

MD, PhD, Assistant Professor, Surgery, Case Western Reserve University School of Medicine, Cleveland, Ohio, United States of America; Chief, Division of Cardiothoracic Surgery Louis Stokes Cleveland VA Medical Center Cleveland, Ohio

BarbarashL. S.BarbarashL. S.

PhD, Professor, Academician of the RAS, chief researcher

PhD, Professor, Academician of the RAS, chief researcher

Research Institute for Complex Issues of Cardiovascular DiseasesРоссияResearch Institute for Complex Issues of Cardiovascular DiseasesRussian FederationHarrington Heart and Vascular Institute, University Hospitals Cleveland Medical CenterСоединённые Штаты АмерикиHarrington Heart and Vascular Institute, University Hospitals Cleveland Medical CenterUnited States201828062018722536Copyright © Antonova L.V., Sevostyanova V.V., Mironov A.V., Krivkina E.O., Velikanova E.A., Matveeva V.G., Glushkova T.V., Elgudin Y.L., Barbarash L.S., 20182018Antonova L.V., Sevostyanova V.V., Mironov A.V., Krivkina E.O., Velikanova E.A., Matveeva V.G., Glushkova T.V., Elgudin Y.L., Barbarash L.S.Antonova L.V., Sevostyanova V.V., Mironov A.V., Krivkina E.O., Velikanova E.A., Matveeva V.G., Glushkova T.V., Elgudin Y.L., Barbarash L.S.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.nii-kpssz.com/jour/article/view/420

Background Currently, the search for the bioactive molecules capable of promoting formation of the vascular tissue is still ongoing. We have previously demonstrated that incorporation of the growth factors and chemoattractant molecules into the biodegradable tubular scaffolds can increase their primary patency upon the implantation into rat abdominal aorta. However, further studies are required to investigate tissue remodeling using functionalized vascular grafts with the same diameter as a replaced native vessel. Aim To investigate the specific aspects of de novo vascular tissue formation and calcification employing rat abdominal aorta interposition model and vascular grafts with 1.5 mm diameter with incorporated vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and stromal cell-derived factor (SDF)-1α. Methods Tubular grafts with a diameter of 1.5 mm were blended of poly(3-hydroxybutyrateco-3-hydroxyvalerate) and poly(ε-caprolactone) (PHBV/PCL). Grafts without growth factors were fabricated using standard electrospinning technique whilst grafts with incorporated growth factors were prepared utilizing emulsion electrospinning. VEGF was incorporated into the inner third, whereas bFGF and SDF-1α were incorporated into the outer two-thirds of the graft. Grafts were implanted into the abdominal aortas of Wistar rats for 1, 3, 6, and 12 months following scanning electron microscopy along with histological and immunofluorescent examination. Results Primary patency of the grafts with VEGF, bFGF, and SDF-1α reached 93% indicative of structural integrity of the vascular tissue. Neither signs of inflammation nor severe calcification was detected. Conclusion As in 2 mm diameter vascular grafts, incorporation of bioactive factors into 1.5 mm diameter grafts increased their long-term primary patency and improved vascular tissue formation in comparison with non-modified grafts.

Background Currently, the search for the bioactive molecules capable of promoting formation of the vascular tissue is still ongoing. We have previously demonstrated that incorporation of the growth factors and chemoattractant molecules into the biodegradable tubular scaffolds can increase their primary patency upon the implantation into rat abdominal aorta. However, further studies are required to investigate tissue remodeling using functionalized vascular grafts with the same diameter as a replaced native vessel. Aim To investigate the specific aspects of de novo vascular tissue formation and calcification employing rat abdominal aorta interposition model and vascular grafts with 1.5 mm diameter with incorporated vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and stromal cell-derived factor (SDF)-1α. Methods Tubular grafts with a diameter of 1.5 mm were blended of poly(3-hydroxybutyrateco-3-hydroxyvalerate) and poly(ε-caprolactone) (PHBV/PCL). Grafts without growth factors were fabricated using standard electrospinning technique whilst grafts with incorporated growth factors were prepared utilizing emulsion electrospinning. VEGF was incorporated into the inner third, whereas bFGF and SDF-1α were incorporated into the outer two-thirds of the graft. Grafts were implanted into the abdominal aortas of Wistar rats for 1, 3, 6, and 12 months following scanning electron microscopy along with histological and immunofluorescent examination. Results Primary patency of the grafts with VEGF, bFGF, and SDF-1α reached 93% indicative of structural integrity of the vascular tissue. Neither signs of inflammation nor severe calcification was detected. Conclusion As in 2 mm diameter vascular grafts, incorporation of bioactive factors into 1.5 mm diameter grafts increased their long-term primary patency and improved vascular tissue formation in comparison with non-modified grafts.

 

 

 

 

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The authors declare that there are no conflicts of interest present.