kpcczКомплексные проблемы сердечно-сосудистых заболеванийComplex Issues of Cardiovascular Diseases2306-12782587-9537Federal State Budgetary Institution “Research Institute for Complex Issues of Cardiovascular Diseases”10.17802/2306-1278-2020-9-2-114-122kpccz-721Research ArticleАНАЛИТИЧЕСКИЙ ОБЗОРANALYTICAL REVIEWПолучение клеточных суспензий артерий при атеросклерозеPreparation of cell suspensionsfrom arteries affected with atherosclerosishttps://orcid.org/0000-0003-2173-2772ШарышД. В.SharyshD. V.

Шарыш Диана Владимировна - аспирант, лаборант-исследователь лаборатории.

Набережная реки Ушайки, 10, Томск, 634050

Sharysh Diana V. - PhD student, laboratory assistant.

10, Ushayka embankment, Tomsk, 634050

sharysh.diana@gmail.comhttps://orcid.org/0000-0002-5824-6439МарковА. В.MarkovA. V.

Марков Антон Владимирович - кандидат медицинских наук, научный сотрудник лаборатории.

Набережная реки Ушайки, 10, Томск, 634050

Markov Anton V. - PhD, researcher.

https://orcid.org/0000-0002-0673-4094НазаренкоМ. С.NazarenkoM. S.

Назаренко Мария Сергеевна - доктор медицинских наук, руководитель лаборатории.

Набережная реки Ушайки, 10, Томск, 634050

Nazarenko Mariya S. - PhD, Head of the Laboratory.

10, Ushayka embankment, Tomsk, 634050

Научно-исследовательский институт медицинской генетики федерального государственного бюджетного научного учреждения Томский национальный исследовательский медицинский центр Российской академии наукРоссияResearch Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of SciencesRussian Federation20202406202092114122Copyright © Шарыш Д.В., Марков А.В., Назаренко М.С., 20202020Шарыш Д.В., Марков А.В., Назаренко М.С.Sharysh D.V., Markov A.V., Nazarenko M.S.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.nii-kpssz.com/jour/article/view/721

Ключевым этапом в исследованиях, направленных на изучение роли отдельных типов клеток в функционировании артерий человека in vivo как в норме, так и при поражении атеросклерозом, является получение суспензии клеток из стенки кровеносного сосуда. К настоящему времени разработан ряд подходов к дезагрегации артерий. В обзоре систематизированы опубликованные ранее данные и методики, представлен алгоритм оптимизации процедуры дезагрегации артерий, а также описаны возникающие проблемы и способы их решения.

Preparation of cell suspension from arterial walls is a key issue in planning the research aimed at studying the role of individual cell types in human arteries in vivo, both normal and affected with atherosclerosis. To date, there is a range of approaches for disaggregating arterial tissues. In this review, we report previously published data and techniques for disaggregating arterial tissues. In addition, an algorithm able optimizing the disaggregation of arteries is proposed taking into account emerging problems and probable solutions.

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The authors declare that there are no conflicts of interest present.