EXPRESSION OF GENES OF BIOMOLECULES ASSOCIATED WITH THE ETIOPATHOGENESIS OF ATHEROSCLEROSIS IN ATHEROSCLEROTIC PLAQUES OF CORONARY ARTERIES
Sergey S. Semaev,
Elena V. Shakhtshneider,
Dinara E. Ivanoshchuk,
Veniamin S. Fishman,
Yana V. Polonskaya,
Elena V. Kashtanova,
Alexander M. Chernyavskiy,
Ivan S. Murashov,
Alexander M. Volkov,
Yulia I. Ragino https://doi.org/10.17802/2306-1278-2023-12-4S-65-79
Abstract
Highlights
The study showed differences in the expression of a number of genes in atherosclerotic plaques of different types in patients with coronary atherosclerosis. The obtained data can become the basis for the development of test systems in order to determine the dynamics of the atherosclerotic process and detect signs of destabilization of the atherosclerotic plaque as early as possible.
Aim. To study the differential expression of genes encoding molecules associated with the etiopathogenesis of atherosclerosis by the method of genome-wide RNA sequencing in stable atherosclerotic plaque of fibrous type and unstable atherosclerotic plaque of dystopic-necrotic type.
Methods. The study was performed on samples of atherosclerotic plaques of patients with coronary atherosclerosis without acute coronary syndrome with stable angina pectoris of functional class II–IV (FC) at the age of 45-65 years. Tissue sampling of atherosclerotic plaques was performed intraoperatively in the presence of indications. Genome-wide RNA sequencing was performed using Illumina’s TruSeq RNA Sample Preparation Kit (Illumina, USA).
Results. An increase in the level of gene expression in stable atherosclerotic plaques was noted for A2M, ADAMTS13, CSF3, CX3CL1, CXCL1, FGF2, GDF15, ICAM1, IL1A, IL1B, IL6, IL10, PDGFA, PTX3. There was an eightfold statistically significant increase in the level of CFD, CXCL16, FABP4, FLT3, IFNG, IL7, IL15, SELL, TGFA, THBD, TNNT1, VCAM1 and VEGFA gene expression (p<0,001) in unstable atherosclerotic plaques of dystrophic-necrotic type.
Conclusion. The study showed differences in the expression of a number of genes in atherosclerotic plaques of different types in patients with coronary atherosclerosis. The obtained data can become the basis for the development of test systems in order to determine the dynamics of the atherosclerotic process and detect signs of destabilization of the atherosclerotic plaque as early as possible.
About the Authors
Sergey S. Semaev
The Institute of Internal and Preventive Medicine – a branch of a Federal Publicly Funded Institution “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”;
“Institute of Cytology and Genetics”, Siberian Branch of the Russian Academy of Sciences;
Russian Federation
Russian Federation
Junior Researcher at the Laboratory of Molecular Genetic Investigations of Therapeutic Diseases, Institute of Internal and Preventive Medicine – a branch of a Federal Publicly Funded Institution “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”, Novosibirsk, Russian Federation; Junior Researcher at the Laboratory of the Study of Monogenic Forms of Common Human Diseases, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation
Elena V. Shakhtshneider
The Institute of Internal and Preventive Medicine – a branch of a Federal Publicly Funded Institution “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”;
“Institute of Cytology and Genetics”, Siberian Branch of the Russian Academy of Sciences;
Russian Federation
Russian Federation
PhD, MD, Leading Researcher at the Institute of Internal and Preventive Medicine – a branch of a Federal Publicly Funded Institution “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”, Novosibirsk, Russian Federation; Head of the Laboratory of the Study of Monogenic Forms of Common Human Diseases, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation
Dinara E. Ivanoshchuk
The Institute of Internal and Preventive Medicine – a branch of a Federal Publicly Funded Institution “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”;
“Institute of Cytology and Genetics”, Siberian Branch of the Russian Academy of Sciences;
Russian Federation
Russian Federation
Researcher at the Laboratory of Molecular Genetic Investigations of Therapeutic Diseases, Institute of Internal and Preventive Medicine – a branch of a Federal Publicly Funded Institution “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”, Novosibirsk, Russian Federation; Junior Researcher at the Laboratory of Human Molecular Genetics, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation
Veniamin S. Fishman
“Institute of Cytology and Genetics”, Siberian Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
PhD, Leading Researcher, Head of the Laboratory of Genomic Mechanisms of Ontogenesis, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation
Yana V. Polonskaya
The Institute of Internal and Preventive Medicine – a branch of a Federal Publicly Funded Institution “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”
Russian Federation
Russian Federation
PhD, Senior Researcher at the Laboratory of Clinical, Biochemical and Hormonal Studies of Therapeutic Diseases, Institute of Internal and Preventive Medicine – a branch of a Federal Publicly Funded Institution «Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences», Novosibirsk, Russian Federation
Elena V. Kashtanova
The Institute of Internal and Preventive Medicine – a branch of a Federal Publicly Funded Institution “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”
Russian Federation
Russian Federation
PhD, Senior Researcher, Head of the Laboratory of Clinical, Biochemical and Hormonal Studies of Therapeutic Diseases, Institute of Internal and Preventive Medicine – a branch of a Federal Publicly Funded Institution “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”, Novosibirsk, Russian Federation
Alexander M. Chernyavskiy
Federal State Budgetary Institution “National Medical Research Center named after academician E.N. Meshalkin” of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
MD, Professor, Corresponding Member of the Russian Academy of Sciences, Honored Scientist of the Russian Federation, Head of the Federal State Budgetary Institution “National Medical Research Center named after academician E.N. Meshalkin” of the Ministry of Health of the Russian Federation, Novosibirsk, Russian Federation
Ivan S. Murashov
Federal State Budgetary Institution “National Medical Research Center named after academician E.N. Meshalkin” of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
Researcher at the Laboratory of Pathomorphology, Federal State Budgetary Institution “National Medical Research Center named after academician E.N. Meshalkin” of the Ministry of Health of the Russian Federation, Novosibirsk, Russian Federation
Alexander M. Volkov
Federal State Budgetary Institution “National Medical Research Center named after academician E.N. Meshalkin” of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
MD, Professor, Head of the Laboratory of Pathomorphology, Federal State Budgetary Institution “National Medical Research Center named after academician E.N. Meshalkin” of the Ministry of Health of the Russian Federation, Novosibirsk, Russian Federation
Yulia I. Ragino
The Institute of Internal and Preventive Medicine – a branch of a Federal Publicly Funded Institution “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”
Russian Federation
Russian Federation
MD, Professor, Corresponding Member of the Russian Academy of Sciences, Head of the Institute of Internal and Preventive Medicine – a branch of a Federal Publicly Funded Institution “Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences”, Novosibirsk, Russian Federation
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Supplementary files
Review
For citations:
Semaev S.S., Shakhtshneider E.V., Ivanoshchuk D.E., Fishman V.S., Polonskaya Ya.V., Kashtanova E.V., Chernyavskiy A.M., Murashov I.S., Volkov A.M., Ragino Yu.I. EXPRESSION OF GENES OF BIOMOLECULES ASSOCIATED WITH THE ETIOPATHOGENESIS OF ATHEROSCLEROSIS IN ATHEROSCLEROTIC PLAQUES OF CORONARY ARTERIES. Complex Issues of Cardiovascular Diseases. 2023;12(4S):65-79. (In Russ.) https://doi.org/10.17802/2306-1278-2023-12-4S-65-79
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