ANALYSIS OF THE PERFUSION PROPERTIES OF THE SCAFFOLD

Abstract. A scaffold usage for the cell culturing is a widespread tissue engineering approach, including culturing in a bioreactor, however the effective use of a bioreactor requires adaptation of the scaffold at the stage of its design. In our opinion, this means assessment of the perfusion properties of the scaffold.

Aim of the study: Aim of the study was the assessment of the perfusion properties of a scaffold sheet made of 11% w/v polycaprolactone (PCL) composite material with gelatinization of 0.5%, 2%, and 4% w/v and fabricated with different electrospinning parameters and hydrostatic pressure (5, 10, and 15 mm Hg).

Materials and methods: To produce composite material, polycaprolactone, gelatin (type B), and solvents: 1,1,1,3,3,3-hexafluoroisopropanol, 2,2,2-trifluoroethanol and chloroform were used. Three composite variants of 11% w/v PCL with gelatinization of 0.5%, 2%, and 4% w/v were prepared. Perfusion properties of the scaffold sheet were assessed with an original experimental equipment. Structural analysis was carried out using the laser confocal microscopy.

Results: Polymer scaffold sheet fabricated with electrospinning demonstrated the possibility of the transverse and longitudinal perfusion through the sheet under hydrostatic pressure of 5, 10, and 15 mm Hg. Also was found what time of 1 mL saline transverse and longitudinal perfusion through a PCL electrospun sheets depends from gelatinization and electrospinning parameters (transverse perfusion time of the sheet fabricated with the electrospinning collector speed of 450 rpm was 10 times lower than sheets fabricated with speed 90-180 rpm), with equal samples thickness and perfusion pressure.

Conclusion: An obtained results allows to optimize the polymer scaffold fabrication process with electrospinning to achieve the optimal characteristics of the perfusion through the scaffold. 

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