COMPOSITE CONTRAST USING TO RESEARCH BIOLOGICAL OBJECTS BY SCANNING ELECTRON MICROSCOPY
https://doi.org/10.17802/2306-1278-2017-6-3-93-103
Abstract
The purpose. Мodification of scanning electron microscopy method in backscattered electrons to research biological samples with massive metal or mineral inclusions.
Materials and methods. In the article the alternative method without cutting sections were supplied. Samples for researching (plates from NiTi surrounded by tissues after subcutal implantation, explanted stents, explanted epoxy covered xenoaortic bioprosthetic heart valves , explanted biodegradable vascular grafts from rats abdominal aorta) were embedded in epoxy risin, polymerized, made surface slices from blocks with samples, contrasted by uranyl acetate and lead citrate and researched in backscattered electrons.
Results. By using the proposed method samples acquired composite contrast and subcellular structures had good visualization in scanning electron microscopy.
Conclusion. Our research showed availability this method to research microstructural characteristics of contacts between hard material and biological tissues and to analyze cellular structure on implant- tissue border. Besides, this method is universal for researching different biological samples, and it has special advantages for research materials with volumetric metal and mineral inclusions. Another advantage of this method is ability to research samples with large square of surface, what can’t be made by using transmission electron microscopy method.
About the Authors
P. A. Mukhamadiarov
Federal State Budgetary Scientific Institution “Research Institute for Complex Issues of Cardiovascular Diseases”
Russian Federation
Kemerovo
Russian Federation
Kemerovo
V. V. Sevostianova
Federal State Budgetary Scientific Institution “Research Institute for Complex Issues of Cardiovascular Diseases”
Russian Federation
Kemerovo
Russian Federation
Kemerovo
D. K. Shishkova
Federal State Budgetary Scientific Institution “Research Institute for Complex Issues of Cardiovascular Diseases”
Russian Federation
Kemerovo
Russian Federation
Kemerovo
M. V. Nasonova
Federal State Budgetary Scientific Institution “Research Institute for Complex Issues of Cardiovascular Diseases”
Russian Federation
Kemerovo
Russian Federation
Kemerovo
S. F. Zinchuk
Federal State Budgetary Educational Institution of higher professional education Kemerovo state medical university
Russian Federation
Kemerovo
Russian Federation
Kemerovo
U. A. Kudryavceva
Federal State Budgetary Scientific Institution “Research Institute for Complex Issues of Cardiovascular Diseases”
Russian Federation
Kemerovo
Russian Federation
Kemerovo
References
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Review
For citations:
Mukhamadiarov P.A., Sevostianova V.V., Shishkova D.K., Nasonova M.V., Zinchuk S.F., Kudryavceva U.A. COMPOSITE CONTRAST USING TO RESEARCH BIOLOGICAL OBJECTS BY SCANNING ELECTRON MICROSCOPY. Complex Issues of Cardiovascular Diseases. 2017;(3):93-103. (In Russ.) https://doi.org/10.17802/2306-1278-2017-6-3-93-103
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