COMPARING BIORESORPTION OF PLAIN AND CELL-LOADED POLYHYDROXYALKANOATE AND POLYCAPROLACTONE SCAFFOLDS POTENTIALLY SUITABLE FOR SMALL HYBRID VASCULAR GRAFTS PRODUCTION
https://doi.org/10.17802/2306-1278-2012-1-26-29
Abstract
Purpose. The study was aimed at evaluating the impact of multipotent mesenchymal stromal cells derived from the marrow (MMSC) on the biodegradation speed of polyhydroxyalkanoate and polycaprolactone copolymer compositions potentially suitable for small vascular graft production. Materials and methods. The study compared fi lm scaffold (FS) № 1 made from 5 % polyhydroxybutirate and valerate and 10 % polycaprolactone (PCL) and fi lm scaffold (FS) № 2 made from 7,5 % polyhydroxybutirate and valerate and 10 % PCL. MMSC were derived from Wistar rat femoral bones. Results. The experiment showed that FS № 2 resorption started 2 months earlier than that of FS № 1 due to higher percentage of polyhydroxybutirate and valerate. Conclusions. The presence of MMSC delayed the start of copolymer scaffold bioresorption for 1 month and reduced the duration inflammatory response in the surrounding tissues.
About the Authors
L. V. Antonova
Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo
Russian Federation
Russian Federation
A. Y. Burago
Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo
Russian Federation
Russian Federation
V. G. Matveeva
Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo
Russian Federation
Russian Federation
Y. G. Toropova
Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo
Russian Federation
Russian Federation
E. A. Velikanova
Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo
Russian Federation
Russian Federation
Yu. A. Kudryavtseva
Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo
Russian Federation
Russian Federation
M. V. Nasonova
Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo
Russian Federation
Russian Federation
A. S. Golovkin
Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo
Russian Federation
Russian Federation
References
1. Шишацкая Е. И. Клеточные матриксы из резорбируемых полигидроксиалканоатов // Клеточная трансплантология и тканевая инженерия. 2007. Т. II, № 2. С. 68–72.
2. Characterization of nonexpanded mesenchymal progenitor cells from normal adult human bone marrow / N. Boiret [et al.] // Exp. Hematol. 2005. Vol. 33. P. 219–225.
3. Devine S. M., Hoffman R. Role of mesenchymal stem cells in hematopoietic stem cell transplantation // Curr. Opin. Hematol. 2000. Vol. 7. P. 358–363.
4. Fabrication and characterization of six electrospun poly(alpha-hydroxy ester) based fi brous scaffolds for tissue engineering / W. J. Li [et al.] // Acta Biomaterialia. 2006. № 2. Р. 377–385.
5. Factorial design optimization and in vivo feasibility of poly(e-caprolactone)-microand nanofi ber-based small diameter vascular grafts / B. Nottelet [et al.] // J. of Biomedical Materials Research. 2008. Part A. P. 865–875.
6. In vitro and in vivo degradation of non-woven materials made of poly(e-caprolactone) nanofi bers prepared by electrospinningunder different conditions / N. Bolgen [et al.] // J. Biomater. Scin. Polym. Ed. 2005. № 16. P. 1537–1555.
7. Shin’oka T., Imai Y., Ikada Y. Transplantation of a Tissue-Engineered Pulmonary Artery // N. Engl. J. Med. 2001. T. 344. P. 532–533.
8. Siegel G., Shaffer R., Dazzi F. The immunosupressive properties of mesenchymal stem cells // Transplantation. 2009. Vol. 87, № 9. P. 45–49.
9. The role of mesenchymal stem cells in haemopoiesis / F. Dazzi [et al.] // Вlood Rev. 2006. Vol. 20. P. 161–171.
10. The T-box transcription factor Brachury mediates cartilage development in mesenchymal stem cell line C3H10T1/2 / A. Hoffman [et al.] // J. Cell. Sci. 2002. Vol. 115. P. 769–781.
11. Webb A. R., Yang J., Ameer G. A. Biodegradable polyester elastomers in tissue engineering // Expert Opin. Biol. Ther. 2004. Vol. 4(6). P. 801–812.
Review
For citations:
Antonova L.V., Burago A.Y., Matveeva V.G., Toropova Y.G., Velikanova E.A., Kudryavtseva Yu.A., Nasonova M.V., Golovkin A.S. COMPARING BIORESORPTION OF PLAIN AND CELL-LOADED POLYHYDROXYALKANOATE AND POLYCAPROLACTONE SCAFFOLDS POTENTIALLY SUITABLE FOR SMALL HYBRID VASCULAR GRAFTS PRODUCTION. Complex Issues of Cardiovascular Diseases. 2012;(1):26-29. (In Russ.) https://doi.org/10.17802/2306-1278-2012-1-26-29
Views:
463
