EVALUATION OF CARDIOTOXICITY OF HYDROLYTIC DEGRADATION PRODUCTS OF TUBULAR POLYMER MATRICES SUITABLE FOR SMALL DIAMETER VASCULAR GRAFTS

The evaluation of cardiotoxicity of the degradation products of electrospunpolyhydroxybutyrate-valerate and polycaprolactone tubular matrices, subjected to 6-month hydrolytic degradation in sterile phosphate buffered saline (PBS) at 37 ºC, was performed. After matrices incubation in PBS, high performance liquid chromatography was used to analyze individual analytes for the presence of monomers and other compounds each month. Cardiotoxicity of analytes was evaluated in a model of isolated perfused rat heart (male Wistar rats) using the Langendorff technique. The rate of coronary flow and enzymatic activity of creatine phosphokinase and lactate dehydrogenase myocardial fractions were measured during the analytes injections. Both, polyhydroxybutyrate-valerate and polycaprolactone, had no significant signs of hydrolytic degradation of polymers into monomers after 6-month storage time. PBS after 6-month incubation of the studied samples did not produce any cardiotoxic effect in the experiments on isolated rat heart and did not demonstrate any significant increase of enzyme activity in the perfusate.

bioresorbable polymers, hydrolytic degradation, chromatography, cardiotoxicity, creatine phosphokinase, lactate dehydrogenase

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