INVESTIGATION OF KETAMINE EFFECTS ON A CELLULAR MODEL OF THE NEUROVASCULAR UNIT AND ITS NEUROPROTECTIVE ROLE IN CHILDREN UNDERGOING CARDIAC SURGERY

Highlights
This article is devoted to the study of the effect of ketamine on the cellular model of the neurovascular unit in modeling conditions similar to cardiac surgery.

Aim. To reveal the effects of ketamine on the cell model of the neurovascular unit within hypoxia and the impact of IL-6.

Methods. An in vitro neurovascular unit (NVE) model consisting of neurons, astrocytes, and endotheliocytes has been formed. The normoxia conditions were the oxygen fraction in the medium of 20%, hypoxia – 4%. To identify the effect of ketamine on the culture of the neurovascular unit, cells were cultured with serum obtained from three groups of patients at different points: after ketamine infusion for 16 hours at a dose of 0.1 mg/kg/hour, at a dose of 0.3 mg/kg/hour, and from patients who did not receive ketamine infusion. All three groups of cells were further cultured under conditions of normoxia and hypoxia.

Results. The fluorescence intensity of occludin-1 and claudin-5 did not differ between the groups with different ketamine levels and the control group, and there were no changes under hypoxic conditions. The fluorescence intensity for the expression of interleukin-1 and interleukin-6 receptors decreased in the ketamine group, and the effect increased with increasing ketamine dose. Under hypoxic conditions, the decrease in fluorescence intensity was more significant.

Conclusion. The effect of ketamine on NVU cells in an in vitro experiment did not significantly affect the expression of tight junction proteins under conditions of normoxia and hypoxia, however, it led to decreasing expression of interleukin receptors, which was more significant after exposure to hypoxia on NVU cells.

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