ABILITY OF N(2)-L-ALANYL-L-GLUTAMINE TO RESTORE THE FUNCTION OF ISCHEMIC MYOCARD

The purpose. The goal of this study was to assess the revitalizing effects of N(2)-L-alanyl-L-glutamine («Dipeptiven») on ischemic heart rate.

Materials and methods. Isolated hearts of laboratory rats were perfused by Langendorff method (n=7). After global cardioplegic ischemia hearts of experimental group were perfused by standard solution with N(2)-L-alanyl-L-glutamine. Control group was excluded from the pharmaceuticals on reperfusion. Physiological indices of the hearts were fixed. Classic and highly specific markers of myocardial damage were studied by using biochemistry methods and ELISA. NADH dynamics in the myocardial tissue were also recorded.

Main Results. Levels of troponin I and NO were significantly lower than baseline and control values. Changes in intracellular translocation enzymes fluctuated at low values and were not significantly different from control during reperfusion, but significantly higher than the original translocation. Level of H-FABP and formation of peroxides were significantly higher in compares with original values. Respiratory chain of both groups was overloaded by electrons during the ischemia.

Conclusions. The structure of contractile proteins was stabilized by reperfusion solution with N(2)-L-alanyl-Lglutamine. However, protection of cardiomyocytes was ineffective from oxidative stress. «Dipeptiven» should be used in combination with other pharmaceuticals.

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