Overview of existing in vitro BBB models: advantages and disadvantages, current state and future prospects
https://doi.org/10.17802/2306-1278-2021-10-3-109-120
Abstract
There is growing research focusing on endothelial cells as separate units of the blood-brain barrier (BBB), and on the complex relationships between different types of cells within a neurovascular unit. To conduct this type of studies, researches use vastly different in vitro BBB models. The main objective of such models is to study the BBB permeability for different molecules, and to advance the current level of understanding the mechanisms of disease and to develop methods of targeted therapy for the central nervous system. The analysis of the existing Abstract in vitro BBB models and their advantages/disadvantages was conducted using the clinical trial data obtained in Russian/foreign countries. In this review, the authors highlight the most relevant assessment parameters and propose a unified classification of in vitro BBB models. According to the performed analysis, there is a tendency to move from 2D BBB models based on semipermeable inserts to 3D BBB spheroid and microfluidic organ-on-chip models. Moreover, the use of human induced pluripotent stem cells instead of animal primary cells will make it possible to reliably scale the results obtained in vitro to conditions in vivo.
About the Authors
A. I. Mosiagina
Federal State Budgetary Educational Institution of Higher Education Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky of the Ministry of Healthcare of the Russian Federation
Russian Federation
Competing Interests:
Russian Federation
Mosiagina Angelina I., MD, PhD student at the Department of Biological Chemistry, Junior Researcher at the Research Institute of Molecular Medicine and Pathobiochemistry.
1, Partizana Zheleznyaka St., Krasnoyarsk, 660022
Competing Interests:
А.И. Мосягина заявляет об отсутствии конфликтов интересов
A. V. Morgun
Federal State Budgetary Educational Institution of Higher Education Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky of the Ministry of Healthcare of the Russian Federation
Russian Federation
Competing Interests:
Russian Federation
Morgun Andrey V., MD, PhD, Associate Professor at the Department of Pediatrics, Institute of Postgraduate Education, Chair, Department of Pediatrics.
1, Partizana Zheleznyaka St., Krasnoyarsk, 660022
Competing Interests:
А.В. Моргун заявляет об отсутствии конфликтов интересов
A. B. Salmina
Federal State Budgetary Educational Institution of Higher Education Krasnoyarsk State Medical University named after Professor V.F. Voino-Yasenetsky of the Ministry of Healthcare of the Russian Federation; Research Center of Neurology
Russian Federation
Competing Interests:
Russian Federation
Salmina Alla B., MD, PhD, Professor, Leading Researcher at the Research Institute of Molecular Medicine and Pathobiochemistr, Krasnoyarsk State MU named after Professor V.F. Voino-Yasenetsky; Leading Researcher, Head of the Laboratory of Experimental Neurocytology, Department of Brain Research, Research Center of Neurology.
1, Partizana Zheleznyaka St., Krasnoyarsk, 660022; 80, Volokolamskoye Hwy, Moscow, 125367
Competing Interests:
А.Б. Салмина входит в редакционную коллегию журнала «Комплексные проблемы сердечно-сосудистых заболеваний»
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Review
For citations:
Mosiagina A.I., Morgun A.V., Salmina A.B. Overview of existing in vitro BBB models: advantages and disadvantages, current state and future prospects. Complex Issues of Cardiovascular Diseases. 2021;10(3):109-120. (In Russ.) https://doi.org/10.17802/2306-1278-2021-10-3-109-120
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