MORPHOFUCTIONAL FEATURES OF AORTIC GRAFT AFTER DECELLULARIZATION
https://doi.org/10.17802/2306-1278-2012-2-3-6
Abstract
Purpose. The aim is to analyze the features of aortic valve allografts decellularized with trypsin solution or a combination of the sodium dodecyl sulfate/deoxycholate solution.
Materials and methods. We performed histological, bacteriological and mechanical testing of graft.
Results. It was found that after the removal of cellular elements with trypsin the strength of allograft significantly decreased compared with the control group and the samples exposed to detergent treatment. Histological analysis of aortic allograft specimens showed signifi cantly impaired spatial organization of the connective tissue framework of the valve after the enzymatic treatment.
Conclusion. Detergent decellularization is the preferred method for obtaining cell-free aortic allografts because it can effectively remove the donor cells and maintain the microstructure and biomechanical properties of the valve graft.
About the Authors
D. S. Sergeevichev
Academician E. N. Meshalkin Research Institute for Circulatory Pathology, Novosibirsk
Russian Federation
Russian Federation
N. A. Podhvatilina
Academician E. N. Meshalkin Research Institute for Circulatory Pathology, Novosibirsk
Russian Federation
Russian Federation
M. B. Vasilieva
Academician E. N. Meshalkin Research Institute for Circulatory Pathology, Novosibirsk
Russian Federation
Russian Federation
N. R. Nichay
Academician E. N. Meshalkin Research Institute for Circulatory Pathology, Novosibirsk
Russian Federation
Russian Federation
A. I. Subbotovskaya
Academician E. N. Meshalkin Research Institute for Circulatory Pathology, Novosibirsk
Russian Federation
Russian Federation
A. M. Karaskov
Academician E. N. Meshalkin Research Institute for Circulatory Pathology, Novosibirsk
Russian Federation
Russian Federation
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Review
For citations:
Sergeevichev D.S., Podhvatilina N.A., Vasilieva M.B., Nichay N.R., Subbotovskaya A.I., Karaskov A.M. MORPHOFUCTIONAL FEATURES OF AORTIC GRAFT AFTER DECELLULARIZATION. Complex Issues of Cardiovascular Diseases. 2012;(2):3-6. (In Russ.) https://doi.org/10.17802/2306-1278-2012-2-3-6
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